Isolation, Purification and Characterization of Antimicrobial Peptides

Research center: Department of Biotechnology, DAV College, Sector- 10, Chandigarh

Supervisor: Dr. Kakoli Biswas Associate Professor , Department of Biotechnology , DAV College, Sector- 10, Chandigarh

Co- supervisor: Dr. Rajesh Biswas, Associate Professor, Department of Zoology, Govt. Home Science College, Sector-10, Chandigarh

Research Scholar: Samriti

Enrolment No. and Date: 14/322/Ph.D. 15020/R&S; 28 November, 2014

Tentative/ Approved Title: Isolation, Purification and Characterization of Antimicrobial Peptides from selected Indian medicinal plants


Antimicrobial peptides (AMPs) are the crucial factors, which plays an important role in host defense mechanisms. AMPs are ubiquitous and found in diverse organisms ranging from microorganisms to animals. Plants are the precious source of natural antimicrobial molecules including antimicrobial peptides known as plant antimicrobial peptides (PAMPs). PAMPs can be divided into different families based on their, molecular weight, activity against different microbes, structure, charge of molecules, content of disulphide bond and mechanism of action. Based on number of cysteine residues and disulfide bonds, PAMPs are categorized into six main families. These peptides may lead to degradation of nutrients that are specific or essential for microbes to grow, interfering with microbial membrane or by conflicting with their metabolism. PAMPs exert multiple antimicrobial activities which includes membrane permeabillization, interference with DNA, RNA and protein synthesis that might provide a suitable approach to prevent bacteria from developing resistance.


  1. Samriti, Biswas. R and Biswas. K, (2018) Plant Antimicrobial Peptides: A novel approach against drug resistant microorganisms. International Journal of Pharmaceutical sciences and Research, Vol. 9   (1): 1000-15.
  2. Samriti, Biswas. R and Biswas. K, (2018) Isolation and characterization of antimicrobial peptides from Datura inoxia leaves having antimicrobial activity against selected bacteria. World journal of pharmaceutical research, Vol. 7(5): 783-794.
  3. Samriti, Biswas. R and Biswas. K, (2019) Antibacterial activity of antimicrobial peptide extracted from Trianthema portulacastrum Leaves. The Pharma Innovation Journal. 8(3): 81-86.